SPECIMEN COLLECTION

SPECIMEN COLLECTION – Definition, Purpose, Principles, Equipment, Procedure for Urine Collection, Urine Culture, Stool-Routine Test and Culture, Sputum Culture, Blood Smear, Blood Culture, Throat Swab, Vaginal Swab/Smear and Urine Testing

DEFINITION

Specimen collection is defined as collection of a required amount of tissue of fluid for laboratory examination

Specimen may be defined as small quantity of a substance, which shows the kind and quality of the whole

PURPOSE

  • To make diagnosis and to help in treatment
  • To note progress or recess of a disease
  • To observe the effects of special treatment and drugs
  • To assess the general health of the patient
  • To investigate the nature of the diseases
  • To aid the doctor in diagnosis and treating the diseases

PRINCIPLE

  • Contaminated and improperly collected specimens will produce false results which will adversely affect the diagnosis and treatment of patient
  • Specimen allowed to stand at room temperature of a long time will give false results due to decomposition of specimen, multiplication of undesirable bacteria and destruction of pathogenic bacteria
  • Blood chemistry is not uniform throughout the day. It varies with the good intake
  • The accuracy and reliability of findings depend upon the correct method collection. Transportation of the specimens to the laboratory and recording of reports
  • Inaccurate results may lead the physician in the diagnosis and treatment of patients
  • Specimens serve as a media for transmission of disease producing organisms to the personnel who handle them carelessly

General Instruction

  • Provide adequate explanations regarding the collection of specimens
  • Ask the patient to wash the external genital area with soap and water then rinse with water alone before collecting urine specimens
  • Equipment used for the collection of specimens should be clean and dry
  • No antiseptic should be present in the specimen bottle
  • As for as possible morning specimens are collected
  • Specimens should be always fresh for the laboratory examination
  • Bacteria multiply in the room temperature so, the specimens which are not tested immediately are kept in the refrigerator, because cold temperature inhibits the growth of bacteria
  • Insist the patient and the personnel to wash hands thoroughly after handing the specimen bottles
  • Container should have a wide mouth to prevent spilling of the specimens, on the outer side of the bottles
  • Containers of the proper size are used according to the nature of specimen

EQUIPMENT

  • Dry container
  • Bed
  • Sterile gauze
  • Artery forceps
  • K-Basin
URINE TESTING 
VAGINAL SWAB/SMEAR
THROAT SWAB
BLOOD CULTURE
BLOOD SMEAR 
SPUTUM CULTURE
STOOL-ROUTINE TEST AND CULTURE 
URINE CULTURE 
PROCEDURE FOR URINE COLLECTION
SPECIMEN COLLECTION - DEFINITION, PRINCIPLE, Instruction, EQUIPMENT
SPECIMEN COLLECTIONDEFINITION, PRINCIPLE, Instruction, EQUIPMENT

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