URINE CULTURE

URINE CULTURE

Collection of midstream specimen of urine without contaminating container and urine specimen

Purpose

  • To collect uncontaminated urine specimen for culture and sensitivity test
  • To detect the microorganism causes urinary tract infection (UTI)
  • To diagnose and treat with specific antibiotic

General Instructions

  • Urine should be collected in sterile containers
  • Urine specimens for culture should be collected in the morning
  • After washing the perineal area and drying collect a midstream specimen of urine in a sterile bottle
  • For bedridden patients cauterized specimen of urine is taken

Preliminary Assessment

Check

  • The doctors order for specific instruction
  • The general condition and diagnoses of the patient
  • Self-care ability of the patient
  • Mental status to follow instructions
  • Articles available in the unit

Preparation of the Patient and Environment

  • Explain the procedure to the patient
  • Provide privacy
  • Arrange the articles at the bedside
  • Collect and arrange the culture bottle – from the laboratory
  • Keep the laboratory request ready

Equipment

  • Basin and soap
  • Towel and sterile gloves
  • Gauze pads 2-3 (if present on strict bed rest)
  • Sterile culture bottle
  • Laboratory request form
  • Sterile K-Basin

Procedure

  • Instruct patient to clean perineum with soap and water
  • Open container and leave cover facing inside up
  • Instruct patient to void into sterile K-Basin. If patient is unable to get out of bed
  • Instruct patient to void directly into sterile container
  • Collect 30-50 ml (1/2 – 3/4  of container) at mid-stream point of voiding
  • Emphasize first and last portions of voiding to the discarded
  • Cap container securely without touching inside of lid

After Care

  • Label container with patients name, hospital number and date
  • Send container to laboratory immediately with lab request
  • Record the procedure in nurse record sheet and register in microbiology laboratory notebook
URINE CULTURE - Purpose, Instructions, Preliminary Assessment, Preparation of the Patient and Environment, Equipment, Procedure, After Care
URINE CULTURE Purpose, Instructions, Preliminary Assessment, Preparation of the Patient and Environment, Equipment, Procedure, After Care

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