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Gloving technique is a crucial aspect of maintaining aseptic conditions in healthcare settings, particularly in sterile environments such as operating rooms or during certain medical procedures. Proper gloving technique helps prevent contamination and ensures the safety of both healthcare professionals and patients.

Medical Gloving

Gloves are used in the medical asepsis to protect the nurse from pathogens. They serve as a barrier when the nurse handles articles contaminated by the feces or wound

Gloves used in the medical asepsis need not to be sterile. These are changed after attending each of the patient discharges. Before wearing the gloves, hand wash, dry and powder them and then put on clean gloves


  • To protect the nurse from pathogens
  • To serve as a barrier when nurses handles contaminated articles
  • To protect the patients with poor resistance
  • It is used for the cleaning for the patient

While Wearing Gloves

  • Wash hands
  • Dry the hands and apply powder for easy insertion of gloves
  • Put on the clean gloves

After Use

  • Remove the gloves and discard them in the container with antiseptic lotion
  • Wash hands thoroughly

General Instructions

  • Every surgical patient should be protected from respiratory, intestinal, skin and other general infections
  • They are to be provided with uncontaminated dishes, bed linen, clothing and other articles

Removing Isolation Clothing

  • Untie the gown at the waist
  • Slip a finger from one hand underneath the cuff of the glove on the opposite hand
  • Pull the glove off, turning inside out as it is removed
  • Deposit in the designed waste receptacle. Repeat the procedure on the opposite hand
  • Remove the mask by untying the ties or slipping the rubber bands from behind the ears. Be careful not to touch the front of the mask. Holding the mask by the ties deposit in the designed waste receptacle
  • Untie the tie at the neck of the gown. Using the ties, pull the gown down toward the front of the body
  • Pull one arm of its sleeve, turning the sleeve inside out as you proceed. Repeat, using the other arm. Be sure the front of the gown does not contact your hands or uniform. Deposit the gown in the designated waste receptacle
  • Wash hands. Put on lab coat



  • Scrub hands using a brush and soap under running water
  • Open the sterile package of gloves with a sterile forceps before scrubbing if no assistant is available
  • After scrubbing, dry hands on a sterile towel
  • Apply sterile powder on both hands
  • The first gloves are drawn on by touching the inside of the gloves cuff
  • The second by placing the gloves hand under the cuff
  • The sterile gown is worn. It should be put on before the gloves so that the glove cuffs may be drawn up over its sleeves
Medical Gloving, Surgical Gloving - Purpose, Procedure, Instructions, After Use
Medical Gloving, Surgical Gloving – Purpose, Procedure, Instructions, After Use

Donning Sterile Gloves:

1. Perform Hand Hygiene:

  • Wash your hands thoroughly with soap and water or use an alcohol-based hand sanitizer to ensure your hands are clean before starting the gloving process.

2. Choose the Correct Glove Size:

  • Select the appropriate size of sterile gloves. Gloves that are too tight may tear, while those that are too loose may compromise dexterity.

3. Open the Outer Glove Package:

  • Grasp the outer package of sterile gloves without touching the inside. Open the package carefully to expose the inner glove package.

4. Put on the First Glove:

  • With the glove package still intact, use the hand opposite to your dominant hand to pick up the glove for your dominant hand.
  • Hold the glove by the folded cuff and insert your dominant hand into the glove without touching the exterior surface.

5. Secure the First Glove:

  • Once the first glove is on, use the gloved hand to pick up the second glove by the inside surface of the cuff.

6. Put on the Second Glove:

  • With the first glove-covered hand, insert your fingers into the second glove without touching the outside of the glove. Pull the second glove over your non-dominant hand.

7. Adjust and Secure Gloves:

  • Adjust the gloves for a comfortable fit, ensuring that they cover the hands and wrists adequately. Secure the cuffs of both gloves to prevent them from rolling down.

8. Perform a Gloving Check:

  • Inspect the gloves for any visible defects, such as tears or punctures. If you notice any issues, remove the gloves, perform hand hygiene, and start the gloving process again.

Doffing Sterile Gloves:

1. Grasp the Outside of the Glove:

  • Pinch the outside of the glove near the wrist with your opposite hand. Avoid touching your skin or clothing.

2. Peel Off the First Glove:

  • Peel off the first glove by turning it inside out as you pull it away from your hand. Hold the removed glove in the gloved hand.

3. Hold the Removed Glove:

  • Keep the removed glove in your gloved hand.

4. Slide Fingers under the Cuff:

  • Slide your fingers from the bare hand under the remaining glove cuff, being careful not to touch the outside of the glove.

5. Peel Off the Second Glove:

  • Peel off the second glove by turning it inside out, covering the first glove. The removed gloves should be inside-out and contained within the remaining glove.

6. Dispose of Gloves:

  • Discard the used gloves into the appropriate waste container without touching the exterior surfaces.

7. Perform Hand Hygiene:

  • Wash your hands thoroughly or use an alcohol-based hand sanitizer after removing the gloves.


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